Part:BBa_K4806222:Design

CYP9Q3 gene with HA-tag for Chlamydomonas reinhardtii (Phytobrick)

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 1991
  Illegal PstI site found at 2171
  Illegal PstI site found at 2677
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 249
  Illegal PstI site found at 1991
  Illegal PstI site found at 2171
  Illegal PstI site found at 2677
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 2533
  Illegal XhoI site found at 530
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 1991
  Illegal PstI site found at 2171
  Illegal PstI site found at 2677
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 1991
  Illegal PstI site found at 2171
  Illegal PstI site found at 2677
  Illegal NgoMIV site found at 2948
  Illegal NgoMIV site found at 3636
  Illegal AgeI site found at 268
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This part has been codon optimized for Chlamydomonas reinhardtii and it is compatible with Phytobricks/MoClo parts.

Source

This composite part is a combination of the following parts:

• 1. AβSAP(i)-promotor (A1-B2) (BBa_K4806013)
• 2. CYP9Q3 gene (B3-B4) (BBa_K4806004)
• 3. HA-tag (B5) (BBa_K3002017)^*
• 4. tRPL23-terminator (B6-C1) (BBa_K3002006)^*

Additionally, connectors were used to create the MoClo overhangs:

• 1. Connector B2-B3 (BBa_K3002303)^*
• 2. Connector B4-B5 (BBa_K3002304)^*
• 3. Connector B5-B6 (BBa_K3002305)^*

The ^* marked parts were not created by us. Our results can be found on the experience page of each part.